FM, along with the imaging device, was evaluated for its performance as an AST tool at the Microbiological Laboratory, Coimbatore, Tamil Nadu, India, which is accredited by National Accreditation Board for Testing and Calibration Laboratories.
Among 440 urine samples tested, 20 were sterile and six contained Candida spies. Both did not quench the fluorescence of SBG medium. Thirty-three samples with a colony count below 1 × 103 CFU/ML (taken as borderline and clinically insignificant) was not detected by FSM within 8 h incubation period. However, the growth and quenching could be detected conveniently on longer and overnight incubation.