Which quenched fluoresce in, a widely-used inexpensive fluoroscope. This quenching of the fluorescence of fluoresce in was directly proportional to the growth of bacterial culture6. Thus, an absence in the fluorescence of the growth medium indicates bacterial growth while the fluorescence of the uncultured medium remains intact. We adapted this bacterial growth monitoring technique that based on the optical shift, for developing a simpler and quicker AST (within 8 h) as reported here. This Fluorescence Quenching Method (FM) is performed in micro-wells of commercially available 12-well strips or plates. The fluorescence of the medium is captured using an optical imager fabricated in-house for 12-well strips.